CD172b / SIRP beta antibody (PE)

Details for Product No. ABIN6559830
Antigen
Reactivity
Human
1
Host
Mouse
1
Clonality (Clone)
Monoclonal ()
Conjugate
PE
Application
Flow Cytometry (FACS)
1
Options
Immunogen NIH-3T3 cells transfected with human CD172b
Clone B4B6
Isotype IgG1
Specificity The mouse monoclonal antibody B4B6 recognizes CD172b, an approximately 50 kDa transmembrane glycoprotein expressed on myeloid cells.
HLDA 8
Cross-Reactivity (Details) Human
Antigen
Background CD172b, the signal-regulatory protein beta (SIRP beta) is a disulfide-linked homodimer expressed on myeloid cells including monocytes and dendritic cells. Similarly to CD172a, it serves as a negative regulator of tyrosine kinase-coupled signaling processes. Unlike CD172a, the CD172b protein does not possess the cytoplasmic domain, but instead its transmembrane domain can interact with another transmembrane protein DAP-12, which contains ITAM sequences in its intracellular domain and links CD172b to the downstream signaling molecules. The result is e.g. regulation of neutrophil transepithelial migration.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.