PSME3 antibody (PSME3)

Details for Product anti-PSME3 Antibody No. ABIN6566551
Antigen
Reactivity
Human, Rat (Rattus)
71
31
25
9
7
5
4
4
4
2
2
2
2
2
2
1
Host
Rabbit
62
9
Clonality
Polyclonal
Conjugate
This PSME3 antibody is un-conjugated
5
3
3
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
57
29
23
11
8
5
5
3
2
1
1
1
1
Options
Immunogen Recombinant protein of human PSME3
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name PSME3 (PSME3 Antibody Abstract)
Background

Synonyms: 11S regulator complex gamma subunit,11S regulator complex subunit gamma,Activator of multicatalytic protease subunit 3,Ki,Ki antigen,Ki nuclear autoantigen,Ki,PA28 gamma,PA28 gamma,PA28g,PA28gamma,Proteasome (prosome,macropain) activator subunit 3 (PA28 gamma,Ki),Proteasome (prosome,macropain) activator subunit 3,Proteasome activator 28 gamma,Proteasome activator 28 subunit gamma,Proteasome activator complex subunit 3,Proteasome activator subunit 3,PSME3,PSME3,REG GAMMA,REG-gamma

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants.

Molecular Weight

Observed_MW: 32kDa

Calculated_MW: 29kDa/30kDa

Gene ID 10197
UniProt P61289
Pathways Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Hepatitis C, Synthesis of DNA
Application Notes WB 1:500 - 1:2000 IF 1:20 - 1:50
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-PSME3 (PSME3) antibody (ABIN6566551) Western blot analysis of extracts of various cell lines, using PSME3 antibody.
Image no. 2 for anti-PSME3 (PSME3) antibody (ABIN6566551) Immunofluorescence analysis of HeLa cells using PSME3 antibody.