DFFA antibody (DNA Fragmentation Factor, 45kDa, alpha Polypeptide)

Details for Product anti-DFFA Antibody No. ABIN6566873
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
167
78
41
2
2
1
1
1
Host
Rabbit
189
31
2
Clonality
Polyclonal
Conjugate
This DFFA antibody is un-conjugated
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
187
74
45
30
27
24
13
11
10
3
3
2
2
1
1
1
Options
Immunogen Recombinant protein of human DFFA
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name DFFA (DFFA Antibody Abstract)
Background

Synonyms: A330085O09Rik,Caspase activated deoxyribonuclease inhibitor short form,DFF 1,DFF 45,DFF alpha,DFF-45,DFF1,DFF35,DFF45,DFFA,Dffa DNA fragmentation factor,alpha subunit,DFFA,DNA fragmentation factor 45 kDa subunit,DNA Fragmentation Factor Alpha Subunit,DNA fragmentation factor subunit alpha,DNA fragmentation factor,45 kD,alpha subunit,DNA fragmentation factor,45  kDa,alpha polypeptide (DFFA),transcript variant 1,DNA fragmentation factor,45  kDa,alpha polypeptide,DNA fragmentation factor,alpha subunit,DNAation factor 45 kDa subunit,H13,ICAD,ICAD L,ICAD S,Inhibitor of CAD,Inhibitor of Caspase Activated DNase,MGC143066,OTTHUMP00000001903,OTTHUMP00000001904,RP23 121D17.3

Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molecular Weight

Observed_MW: 35kDa, 45kDa

Calculated_MW: 29kDa/36kDa

Gene ID 1676
UniProt O00273
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes WB 1:500 - 1:2000 IF 1:50 - 1:100
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody (ABIN6566873) Western blot analysis of extracts of various cell lines, using DFFA antibody.
Image no. 2 for anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibody (ABIN6566873) Immunofluorescence analysis of HeLa cells using DFFA antibody.