DFFA antibody (AA 179-331)
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- Target See all DFFA Antibodies
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
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Binding Specificity
- AA 179-331
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This DFFA antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS)
- Purification
- purified
- Immunogen
- Purified recombinant fragment of human DFFA (AA: 179-331) expressed in E. coli.
- Clone
- 2B10C8
- Isotype
- IgG1
- Top Product
- Discover our top product DFFA Primary Antibody
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- Application Notes
- WB:1:500 - 1:2000, FCM:1:200 - 1:400, ELISA:1:10000,
- Restrictions
- For Research Use only
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- Buffer
- Purified antibody in PBS with 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- 4°C, -20°C for long term storage
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- Target
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- Alternative Name
- DFFA (DFFA Products)
- Synonyms
- DFF-45 antibody, DFF1 antibody, ICAD antibody, A330085O09Rik antibody, DFF35 antibody, Dff45 antibody, ICAD-L antibody, ICAD-S antibody, DFFA antibody, zgc:92229 antibody, DNA fragmentation factor subunit alpha antibody, DNA fragmentation factor, alpha subunit antibody, DNA fragmentation factor, 45kDa, alpha polypeptide antibody, DNA fragmentation factor, alpha polypeptide antibody, DFFA antibody, Dffa antibody, dffa antibody
- Background
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Description: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Aliases: DFF1, ICAD, DFF-45
- Molecular Weight
- 36.5 kDa
- Gene ID
- 1676
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis
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