MRPS12 antibody (Mitochondrial Ribosomal Protein S12)

Details for Product anti-MRPS12 Antibody No. ABIN6566989
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
48
15
10
1
1
1
1
1
1
Host
Rabbit
47
1
Clonality
Polyclonal
Conjugate
This MRPS12 antibody is un-conjugated
3
2
2
2
2
2
1
1
1
1
1
1
Application
Western Blotting (WB)
43
28
20
3
1
1
1
1
Options
Immunogen Recombinant protein of human MRPS12
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name MRPS12 (MRPS12 Antibody Abstract)
Background

Synonyms: 28S ribosomal protein S12,28S ribosomal protein S12 mitochondrial,mitochondrial,Mitochondrial ribosomal protein S12,MRP-S12,MRPS12,MT-RPS12,RPMS12,RPS12,RPSM12,RT12,S12mt

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene, all three variants encode the same protein.

Molecular Weight

Observed_MW: 14kDa

Calculated_MW: 15kDa

Gene ID 6183
UniProt O15235
Pathways Sensory Perception of Sound
Application Notes WB 1:1000 - 1:2000
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-Mitochondrial Ribosomal Protein S12 (MRPS12) antibody (ABIN6566989) Western blot analysis of extracts of various cell lines, using MRPS12 antibody.