SMAD, Mothers Against DPP Homolog 1 (SMAD1) antibody

Details for Product No. ABIN6567043
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
345
223
188
13
12
11
10
10
8
8
7
6
6
6
4
3
2
2
2
2
2
2
2
2
1
Host
Rabbit
326
39
1
Clonality
Polyclonal
Conjugate
Un-conjugated
21
11
9
9
9
9
9
9
7
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
294
158
102
52
38
26
26
17
15
6
5
4
3
2
1
1
1
1
1
Options
Immunogen Recombinant protein of human SMAD1
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name SMAD1 (SMAD1 Antibody Abstract)
Background

Synonyms: BSP-1,BSP1,HsMAD1,JV4-1,JV41,MAD homolog 1,MAD mothers against decapentaplegic homolog 1,Mad related protein 1,Mad-related protein 1,MADH1,MADR1,Mothers against decapentaplegic homolog 1,Mothers against DPP homolog 1,SMA- AND MAD-RELATED PROTEIN 1,SMAD 1,SMAD family member 1,SMAD mothers against DPP homolog 1,Smad1,SMAD1,TGF beta signaling protein 1,Transforming growth factor-beta-signaling protein 1

Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Molecular Weight

Observed_MW: 54kDa

Calculated_MW: 15kDa/52kDa

Gene ID 4086
UniProt Q15797
Pathways Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-SMAD, Mothers Against DPP Homolog 1 (SMAD1) antibody (ABIN6567043) Western blot analysis of extracts of various cell lines, using SMAD1 antibody.
Image no. 2 for anti-SMAD, Mothers Against DPP Homolog 1 (SMAD1) antibody (ABIN6567043) Immunohistochemistry of paraffin-embedded human kidney using SMAD1 antibody.
Image no. 3 for anti-SMAD, Mothers Against DPP Homolog 1 (SMAD1) antibody (ABIN6567043) Immunofluorescence analysis of HeLa cells using SMAD1 antibody.