EIF2S1 antibody (N-Term)

Catalog No. ABIN657392

Product Details anti-EIF2S1 Antibody

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Binding Specificity: AA 60-89, N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: Zf, B, C, D, Rat, Y

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.

Clone: RB33288

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: EIF2S1 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.

Expiry Date: 6 months

References for anti-EIF2S1 antibody (ABIN657392)

Chiu, Yeh, Wang, Huang, Ho, Lin, Wang et al.: "Combination of the novel histone deacetylase inhibitor YCW1 and radiation induces autophagic cell death through the downregulation of BNIP3 in triple-negative breast cancer cells in vitro and in an ..." in: Molecular cancer, Vol. 15, Issue 1, pp. 46, (2017) (PubMed).

So, Chen, Wang, Wu, Huang, Lai, Pan, Kang, Huang: "Midazolam regulated caspase pathway, endoplasmic reticulum stress, autophagy, and cell cycle to induce apoptosis in MA-10 mouse Leydig tumor cells." in: OncoTargets and therapy, Vol. 9, pp. 2519-33, (2016) (PubMed).

Hsu, Simon, Neill, Marcotte, Sayad, Bland, Echeverria, Sun, Kurley, Tyagi, Karlin, Dominguez-Vidaña, Hartman, Renwick, Scorsone, Bernardi, Skinner, Jain, Orellana, Lagisetti, Golding, Jung, Neilson et al.: "The spliceosome is a therapeutic vulnerability in MYC-driven cancer. ..." in: Nature, Vol. 525, Issue 7569, pp. 384-8, (2015) (PubMed).

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1 (EIF2S1 Products)

Background: The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Molecular Weight: 36112

Gene ID: 1965

NCBI Accession: NP_004085

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C