RLTPR antibody (PE)

Catalog No. ABIN6579830

Product Details anti-RLTPR Antibody (PE)

Target: RLTPR (RGD Motif, Leucine Rich Repeats, Tropomodulin Domain and Proline-Rich Containing (RLTPR))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RLTPR antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.

Cross-Reactivity (Details): Human, Mouse

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Murine RLTPR

Clone: EM-53

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for RLTPR

Target: RLTPR (RGD Motif, Leucine Rich Repeats, Tropomodulin Domain and Proline-Rich Containing (RLTPR))

Alternative Name: RLTPR / CARMIL2 (RLTPR Products)

Background: Capping protein regulator and myosin 1 linker 2,RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing, capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 Molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.,CARMIL2, LRRC16C

Gene ID: 146206

UniProt: Q6F5E8