CD86 antibody (APC)

Catalog No. ABIN6655467

Supplier Product No.: 200-326-n75

Product Details anti-CD86 Antibody (APC)

Target: CD86

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 200-326-n75

Supplier: Rockland

Purpose: CD86 Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD86.

Purification: Allophycocyanin conjugated CD86 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD86.

Sterility: Sterile filtered

Immunogen: Anti-CD86 Antibody (Monoclonal) was produced by repeated immunizations with CD86 antigen.

Clone: IT2-2

Isotype: IgG2b kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

Western_Blot_Dilution: User Optimized

Comment:

Anti-CD86 is tested for Flow Cytometry and useful in Immunohistochemistry and Western Blot. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD86

Target: CD86

Alternative Name: CD86 (CD86 Products)

Background:

Synonyms: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, CD86, CD28LG2

Background: CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Gene Name: CD86

Gene ID: 942

NCBI Accession: NP_001193853

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation