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Synaptobrevin (VAMP) antibody Primary Antibody

VAMP Reactivity: Human IHC, WB Host: Mouse Monoclonal SP10
Catalog No. ABIN6656054
$375.00
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100 μL
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  • Target
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Immunohistochemistry (IHC), Western Blotting (WB)
    Cross-Reactivity
    Cow (Bovine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
    Purification
    Anti-Synaptobrevin (VAMP) Antibody is directed against human synaptobrevin. Anti-Synaptobrevin IgM was purified by chromatography. Reactivity is expected against bovine, mouse, pig, rat, rabbit and hamster.
    Immunogen

    Immunogen: Anti-Synaptobrevin (VAMP) Monoclonal Antibody was produced in mouse by repeated immunizations with synaptic immunoprecipitate (crude) from human brain.

    Immunogen Type: Native Protein

    Clone
    SP10
    Isotype
    IgM
  • Application Notes

    Immunohistochemistry Dilution: 1:100

    Application Note: Anti-Synaptobrevin antibody is suitable for use in Western Blotting and IHC. Expect a band of approximately 16 kDa in size corresponding to the VAMP proteins in Western blot of rat brain lysate. Anti-Synaptobrevin antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections. Specific conditions for reactivity should be optimized by the end user. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:1000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

    Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
  • Target
    Background

    Synonyms: Vesicle-associated membrane protein 1

    Background: Synaptobrevin (also VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles. Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer's disease.

    Gene Name: VAMP1/2

    Gene ID
    6843
    UniProt
    P23763
    Pathways
    Tube Formation, Synaptic Vesicle Exocytosis
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