Synaptobrevin (VAMP) antibody Primary Antibody
- Immunohistochemistry (IHC), Western Blotting (WB)
- Cow (Bovine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
- Anti-Synaptobrevin (VAMP) Antibody is directed against human synaptobrevin. Anti-Synaptobrevin IgM was purified by chromatography. Reactivity is expected against bovine, mouse, pig, rat, rabbit and hamster.
Immunogen: Anti-Synaptobrevin (VAMP) Monoclonal Antibody was produced in mouse by repeated immunizations with synaptic immunoprecipitate (crude) from human brain.
Immunogen Type: Native Protein
- Application Notes
Immunohistochemistry Dilution: 1:100
Application Note: Anti-Synaptobrevin antibody is suitable for use in Western Blotting and IHC. Expect a band of approximately 16 kDa in size corresponding to the VAMP proteins in Western blot of rat brain lysate. Anti-Synaptobrevin antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections. Specific conditions for reactivity should be optimized by the end user. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1000
- For Research Use only
Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Synonyms: Vesicle-associated membrane protein 1
Background: Synaptobrevin (also VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles. Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer's disease.
Gene Name: VAMP1/2
- Gene ID
- Tube Formation, Synaptic Vesicle Exocytosis