YWHAB antibody (pSer58)

Catalog No. ABIN6658145

Supplier Product No.: 600-401-d02

Product Details anti-YWHAB Antibody

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Binding Specificity: pSer58

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-d02

Supplier: Rockland

Purpose: 14-3-3 phospho S58 Antibody

Cross-Reactivity (Details): Anti-14-3-3 pS58 antibody is directed against rat 14-3-3 protein phosphorylated at S58.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-14-3-3 pS58 antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Comment:

Anti-14-3-3 pS58 antibody is tested for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to the 14-3-3 proteins phosphorylated at Ser58 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for YWHAB

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Alternative Name: Ywhab (YWHAB Products)

Background:

Synonyms: 14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1

Background: 14-3-3 pS58 antibody detects 14-3-3 protein phosphorylated at S58. 14-3-3 is a member of a family of highly conserved proteins that appear to have multiple roles in cell signaling. 14-3-3 is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders. 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms. 14-3-3 affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation. Anti-14-3-3 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: YWHAB

Gene ID: 56011

NCBI Accession: NP_062250

UniProt: P35213

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location