KCNK3 antibody (AA 251-411) (FITC)

Catalog No. ABIN6658194

Supplier Product No.: 200-302-w85

Product Details anti-KCNK3 Antibody (FITC)

Target: KCNK3 (Potassium Channel, Subfamily K, Member 3 (KCNK3))

Binding Specificity: AA 251-411

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNK3 antibody is conjugated to FITC

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 200-302-w85

Supplier: Rockland

Purpose: TASK1 Potassium Channel Antibody Fluorescein

Cross-Reactivity (Details): BLAST analysis suggests that it is 96 % identical to mouse, 76 % identical to human, and <30 % identical to TASK3.

Purification: Anti-TASK1 Potassium Channel Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-TASK1 Potassium Channel Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 251-411 of rat TASK1.

Clone: S374-48

Isotype: IgG2b

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-TASK1 Potassium Channel FITC Conjugated Antibody is suitable for Western Blots, Immunohistochemistry and Immunocytochemistry. Expect a band approximately ~50kDa on specific lysates or tissues. Does not cross react with TASK3. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for KCNK3

Target: KCNK3 (Potassium Channel, Subfamily K, Member 3 (KCNK3))

Alternative Name: Kcnk3 (KCNK3 Products)

Background:

Synonyms: Potassium channel subfamily K member 3, KCNK3, Acid sensitive potassium channel protein TASK 1, Cardiac two pore background K(+) channel, cTBAK 1, K2p3.1, KCNK9, OAT1, potassium channel subfamily K member 3, rTASK, TASK 1, TBAK1

Background: K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54 % identity with TASK-1, but less than 30 % identity with TASK-2 and other tandem pore K+ channels. Anti-TASK1 Potassium Channel Antibody is ideal for research in Neuroscience, particularly studies concerning the dopaminergic neuron and melatonin signaling.

Gene Name: TASK1

Gene ID: 29553

NCBI Accession: NP_203694

UniProt: O54912