Glutamate Receptor 1 antibody (pSer845)

Catalog No. ABIN6658219

Supplier Product No.: 612-401-c83

The Rabbit Polyclonal anti-Glutamate Receptor 1 antibody has been validated for WB, IHC, ELISA and FM. It is suitable to detect Glutamate Receptor 1 in samples from Rat.

Quick Overview for Glutamate Receptor 1 antibody (pSer845) (ABIN6658219)

Target: Glutamate Receptor 1 (GLUR1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutamate Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Product Details anti-Glutamate Receptor 1 Antibody

Binding Specificity: pSer845

Supplier Product No.: 612-401-c83

Supplier: Rockland

Purpose: GluR1 phospho S845 Antibody

Cross-Reactivity (Details): Anti-GluR1 pS845 Antibody is directed against GluR1 protein phosphorylated at S845.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-GluR1 pS845 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:1000 - 10,000

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:200

Western_Blot_Dilution: 1:1000

Comment:

Anti-GluR1 pS845 antibody is tested for use in IHC and Western Blotting and suitable for IF and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 100 kDa in size corresponding to GluR1 protein phosphorylated at Ser 845 in Western Blots of rat brain extracts.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glutamate Receptor 1

Target: Glutamate Receptor 1 (GLUR1)

Alternative Name: GluR1

Background:

Synonyms: Glutamate receptor 1, GluR1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1,

Background: Anti-GluR1 pS845 antibody recognizes phophorylated GluR1. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR. In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory. Anti-GluR1 pS845 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: GRIA1

Gene ID: 50592

NCBI Accession: NP_113796

UniProt: P19490

Pathways: PI3K-Akt Signaling