GABRG2 antibody (Cytoplasmic Loop)

Catalog No. ABIN6658227

Supplier Product No.: 612-401-c81

Product Details anti-GABRG2 Antibody

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Binding Specificity: Cytoplasmic Loop

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Multiplex Assay (MA)

Supplier Product No.: 612-401-c81

Supplier: Rockland

Purpose: GABA(A) Receptor gamma 2 Antibody

Cross-Reactivity (Details): Anti-GABA(A) Receptor gamma 2 Antibody is directed against rat GABA(A) Receptor gamma 2.

Purification: The antibody was affinity purified from monospecific antiserum by immune affinity purification.

Immunogen: Anti-GABA(A) Receptor gamma 2 Antibody was produced by repeated immunizations with recombinant fusion proteins from the cytoplasmic loop of the gamma 2 subunit of rat GABAA.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Suggested Applications: Other
Anti-GABA(A) Receptor gamma 2 (Rabbit) antibody has been tested in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of 46 kDa in size corresponding to the gamma 2 subunit of the GABA(A) receptor in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GABRG2 antibody (ABIN6658227)

Yamaura, Kiyonaka, Hamachi: "Construction of Protein-Based Biosensors Using Ligand-Directed Chemistry for Detecting Analyte Binding." in: Methods in enzymology, Vol. 589, pp. 253-280, (2017) (PubMed).

Yamaura, Kiyonaka, Numata, Inoue, Hamachi: "Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry." in: Nature chemical biology, Vol. 12, Issue 10, pp. 822-30, (2017) (PubMed).

Target Details for GABRG2

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Alternative Name: GABA(A) Receptor gamma 2 (GABRG2 Products)

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, Gabrg2

Background: Anti-GABA(A) Receptor gamma 2 Antibody detects GABA(A) Receptor gamma 2. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. Anti-GABA(A) Receptor gamma 2 Antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRG2

Gene ID: 29709

NCBI Accession: NP_899156

UniProt: P18508