Dynamin 1 antibody (pSer774)

Catalog No. ABIN6658244

Supplier Product No.: 612-601-d32

Product Details anti-Dynamin 1 Antibody

Target: Dynamin 1 (DNM1)

Binding Specificity: pSer774

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 612-601-d32

Supplier: Rockland

Purpose: Dynamin phospho S774 Antibody

Cross-Reactivity (Details): Anti-Dynamin pS774 antibody is directed against Dynamin.

Purification: Anti-Dynamin pS774 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Dynamin pS774 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Dynamin pS774 Antibody is tested for use in Western Blotting and ICC and suitable in IHC and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protein phosphorylated at Ser774 in the appropriate cell lysate or extract. Anti-Dynamin pS774 antibody labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Anti-Dynamin pS774 antibody does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin (DNM1 Products)

Background:

Synonyms: sheep anti-Dynamin pS774 Antibody, Dynamin-1, B-dynamin, D100

Background: Dynamin Antibody detects Dynamin which is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis. Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo. Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles. Anti-Dynamin pS774 Antibody is ideal for investigators involved in Cell Signaling, Cell Motility, Neuroscience and Cancer Research.

Gene Name: DNM1

Gene ID: 140694

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling