GABRA4 antibody (Cytoplasmic Loop)

Catalog No. ABIN6658273

Supplier Product No.: 612-401-d47

Product Details anti-GABRA4 Antibody

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Binding Specificity: Cytoplasmic Loop

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 612-401-d47

Supplier: Rockland

Purpose: GABA(A) Receptor alpha 4 Antibody

Cross-Reactivity (Details): Anti-GABA(A) Receptor alpha 4 Antibody is directed against rat GABA(A) Receptor alpha 4.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-GABA(A) Receptor alpha 4 Antibody was produced by repeated immunizations with recombinant fusion proteins from the cytoplasmic loop of the alpha 4 subunit of rat GABAA.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-GABA(A) Receptor alpha 4 (Rabbit) antibody is tested for use in Western Blot and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 64 kDa in size corresponding to the alpha 4 subunit of the GABA(A) receptor in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GABRA4

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Alternative Name: GABA(A) Receptor alpha 4 (GABRA4 Products)

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4, Gabra4

Background: Anti-GABA(A) Receptor alpha 4 Antibody detects GABA(A) Receptor alpha 4. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma- subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor. GABA(A) Receptor alpha 4 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRA4

Gene ID: 140675

NCBI Accession: NP_542154

UniProt: P28471

Pathways: Synaptic Membrane