Fibrillarin antibody
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- Target See all Fibrillarin (FBL) Antibodies
- Fibrillarin (FBL)
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Reactivity
- Saccharomyces cerevisiae
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Fibrillarin antibody is un-conjugated
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Application
- Western Blotting (WB), Fluorescence Microscopy (FM)
- Cross-Reactivity
- Human, Mouse (Murine), Rat (Rattus)
- Purification
- Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein. Fibrillarin antibodies are protein G purified from concentrated clarified cell culture supernate. Reactivity is expected against human, mouse, and rat. Cross-reactivity toward other sources has not be determined. Anti-Fibrillarin antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.
- Immunogen
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Immunogen: Anti-Fibrillarin Monoclonal Antibody was produced in mouse by repeated immunizations with native yeast nuclear protein preparations.
Immunogen Type: Native Protein
- Clone
- 38F3
- Isotype
- IgG
- Top Product
- Discover our top product FBL Primary Antibody
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- Application Notes
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Application Note: Anti-Fibrillarin (Mouse) Antibody is suitable for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band size of approximately 34 kDa in size corresponding to the Fibrillarin/Nop1p proteins.
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 1:500
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol
0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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- Target
- Fibrillarin (FBL)
- Alternative Name
- Fibrillarin (FBL Products)
- Synonyms
- FIB antibody, FLRN antibody, RNU3IP1 antibody, wu:fb37g12 antibody, zgc:56145 antibody, zgc:77130 antibody, FBL antibody, AL022665 antibody, CG9888 antibody, Dmel\\CG9888 antibody, Fibri antibody, GCR-6 antibody, GCR6 antibody, Pen59C5 antibody, fib antibody, pen59C5 antibody, MGC76139 antibody, fbl antibody, fibrillarin antibody, Fibrillarin antibody, putative fibrillarin antibody, fibrillarin S homeolog antibody, FBL antibody, fbl antibody, Fbl antibody, Fib antibody, fib1 antibody, LMJF_19_0100 antibody, fbl.S antibody
- Background
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Synonyms: mouse anti-Fibrillarin Antibody, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1
Background: Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.
Gene Name: NOP1
- Gene ID
- 851548
- UniProt
- P15646
- Pathways
- Ribonucleoside Biosynthetic Process
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