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Goat anti-Mouse IgG Antibody (Cy2) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Cy2 F(ab')2 fragment
Rockland
Catalog No. ABIN6698833
Supplier Product No.: 710-111-032
  • Target See all IgG products
    IgG
    Fragment
    F(ab')2 fragment
    Reactivity
    • 730
    • 692
    • 596
    • 462
    • 399
    • 240
    • 208
    • 175
    • 145
    • 137
    • 112
    • 107
    • 106
    • 101
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 1761
    • 1547
    • 567
    • 258
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Goat
    Clonality
    • 3390
    • 179
    • 16
    • 1
    Polyclonal
    Conjugate
    • 591
    • 421
    • 421
    • 378
    • 274
    • 262
    • 235
    • 118
    • 106
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 50
    • 44
    • 43
    • 40
    • 39
    • 39
    • 37
    • 35
    • 35
    • 32
    • 31
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    Cy2
    Application
    • 2059
    • 1985
    • 1849
    • 1805
    • 998
    • 919
    • 895
    • 487
    • 424
    • 234
    • 234
    • 149
    • 98
    • 84
    • 65
    • 56
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    710-111-032
    Supplier
    Rockland
    Purpose
    F(ab')2 Mouse IgG (H&L) Antibody Cy2 Conjugated Pre-Adsorbed
    Cross-Reactivity (Details)
    Minimal crossreactivity against Bv Hs Hu Rb Rt & Sh Serum Proteins
    Characteristics
    Goat F(ab')2 Anti-Mouse IgG Antibody CY2 Conjugation, Goat Fab2 Anti-Mouse IgG CY2 Conjugated Antibody,F(ab')2 Anti-Mouse IgG (H&L) Cy2 Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
    Purification
    Preadsorption: Pre-Adsorbed
    Immunogen
    Mouse IgG whole molecule
    Isotype
    IgG
    Labeling Ratio
    1.9
  • Application Notes

    FLISA_Dilution: 1:10,000 - 1:50,000

    Flow_Cytometry_Dilution: 1:500 - 1:2,500

    IF_Microscopy_Dilution: 1:1,000 - 1:5,000

    Other: FLOW CYTOMETRY 1:500 - 1:2,500

    Comment

    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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