Goat anti-Human IgA (Chain alpha) Antibody (DyLight 800) - Preadsorbed

Details for Product No. ABIN6698907
Antigen
Epitope
Chain alpha
70
49
22
11
10
6
6
1
1
Reactivity
Human
126
60
38
28
13
11
10
10
10
10
6
5
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
142
88
57
13
7
7
2
Clonality
Polyclonal
Conjugate
DyLight 800
37
34
32
21
20
15
6
5
4
4
4
4
4
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, FLISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Human IgA alpha heavy chain

Isotype IgG
Characteristics

Synonyms: Goat Anti Human IgA (alpha chain) Antibody DyLight 800™Conjugated, Goat Anti-Human IgA (alpha chain) Antibody DyLight 800™Conjugated, Goat Anti Human IgA Antibody DyLight 800™Conjugated

Background: Anti-Human IgA DyLight 800 Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification Preadsorption: Solid phase absorption
Labeling Ratio 2.1
Antigen
Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Human IgA DyLight 800 Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

ELISA Dilution: 1:80,000

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months