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Goat anti-Human IgG Antibody (Cy3.5) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Cy3.5
Rockland
Catalog No. ABIN6698910
Supplier Product No.: 609-112-123
  • Target See all IgG products
    IgG
    Reactivity
    • 686
    • 677
    • 573
    • 433
    • 381
    • 208
    • 195
    • 144
    • 127
    • 126
    • 107
    • 102
    • 96
    • 88
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1705
    • 1431
    • 525
    • 205
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Goat
    Clonality
    • 3130
    • 172
    • 16
    • 1
    Polyclonal
    Conjugate
    • 587
    • 416
    • 415
    • 367
    • 274
    • 262
    • 235
    • 118
    • 85
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 44
    • 39
    • 39
    • 37
    • 35
    • 35
    • 32
    • 31
    • 31
    • 30
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 23
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    • 15
    • 15
    • 15
    • 15
    • 13
    • 10
    • 8
    Cy3.5
    Application
    • 2039
    • 1985
    • 1822
    • 1779
    • 998
    • 919
    • 895
    • 487
    • 424
    • 149
    • 98
    • 64
    • 56
    • 51
    • 45
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    609-112-123
    Supplier
    Rockland
    Purpose
    Human IgG (H&L) Antibody CY3.5 Conjugated Pre-Adsorbed
    Cross-Reactivity (Details)
    Minimal crossreactivity against Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins
    Characteristics
    Goat Anti Human IgG CY3 Conjugated Antibody, Goat Anti-Human IgG Antibody CY3 Conjugation,Anti-Human IgG (H&L) Cy3.5 generated in goat detects human Immunoglobulin G (heavy and light chains).
    Purification
    Preadsorption: Pre-Adsorbed
    Immunogen
    Human IgG whole molecule
    Isotype
    IgG
    Labeling Ratio
    2.9
  • Application Notes

    FLISA_Dilution: 1:10,000 - 1:50,000

    Flow_Cytometry_Dilution: 1:500 - 1:2,500

    IF_Microscopy_Dilution: 1:1,000 - 1:5,000

    Other: FLOW CYTOMETRY 1:500 - 1:2,500

    Comment

    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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