Goat anti-Human IgA, IgG, IgM Antibody (DyLight 800) - Preadsorbed

Details for Product No. ABIN6698943
Antigen
Reactivity
Human
60
33
20
15
11
9
4
4
3
3
3
2
2
1
1
Host
Goat
106
56
4
3
2
Clonality
Polyclonal
Conjugate
DyLight 800
28
27
18
14
9
8
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
ELISA, FLISA, Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Human IgG, IgA, and IgM whole molecules

Isotype IgG
Characteristics

Synonyms: Goat anti Human IgG IgA IgM Antibody DyLight 800™ Conjugation, Goat Anti-Human IgG IgA IgM DyLight 800™ Conjugation Antibody, Goat Anti-Human IgGAM DyLight 800™ Conjugation

Background: Anti-Human IgG IgA IgM (H&L) DyLight 800 Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification Preadsorption: Solid phase absorption
Antigen
Alternative Name iga,igg,igm
Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Human IgG IgA IgM (H&L) DyLight 800 Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

ELISA Dilution: 1:70,000

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months