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Rabbit anti-Mouse IgG (Chain gamma 1), (Chain gamma 2a), (Chain gamma 2b), (Chain gamma 3) Antibody (DyLight 680) - Preadsorbed Secondary Antibody

FLISA, FM, WB Polyclonal IgG DyLight 680
Catalog No. ABIN6698979
$95.00
Plus shipping costs $45.00
100 μg ABIN6698979
100 μg ABIN6698979
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  • Target
    IgG
    Binding Specificity
    Chain gamma 1, Chain gamma 2a, Chain gamma 2b, Chain gamma 3
    • 1531
    • 134
    • 117
    • 53
    • 34
    • 30
    • 25
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Reactivity
    Mouse
    • 367
    • 334
    • 315
    • 224
    • 214
    • 111
    • 110
    • 82
    • 76
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Host
    Rabbit
    • 830
    • 780
    • 312
    • 178
    • 76
    • 75
    • 22
    • 20
    • 16
    • 13
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Clonality
    Polyclonal
    • 1795
    • 177
    Conjugate
    DyLight 680
    Application
    FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
    Characteristics

    Synonyms: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody DyLight™ 680 conjugation, rabbit anti-Mouse IgG DyLight™680 conjugated Antibody

    Background: Anti-Mouse IgG DyLight680 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

    Immunogen Type: Native Protein

    Isotype
    IgG
    Labeling Ratio
    2.6
  • Application Notes

    Application Note: Mouse IgG subclass pan reactive Secondary Antibody is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Rabbit anti-mouse IgG antibody DyLight™ 680 is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Mouse IgG secondary antibody is available in a variety of formats.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgG
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