Rabbit anti-Mouse IgG1 (Heavy Chain) Antibody (DyLight 549)
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- Target See all IgG1 products
- IgG1
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Binding Specificity
- Heavy Chain
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- DyLight 549
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Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-442-040
- Supplier
- Rockland
- Purpose
- Mouse IgG1 (Gamma 1 chain) Antibody DyLight™ 549 Conjugated
- Characteristics
- Rabbit Anti Mouse IgG1 (Gamma 1 chain) Antibody DyLight 549™ Conjugated, Rabbit Anti-Mouse IgG1 Antibody DyLight 549™ Conjugated,Anti-Mouse IgG1 DyLight 549 Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain).
- Immunogen
- Mouse IgG1 heavy chain
- Isotype
- IgG
- Labeling Ratio
- 3.1
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- Application Notes
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- Comment
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgG1
- Abstract
- IgG1 Products
- Target Type
- Antibody
- Background
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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