Rabbit anti-Mouse (Murine) IgG3 (Chain gamma 3) Antibody (DyLight 549) - Preadsorbed

Details for Product No. ABIN6699079
Antigen
Epitope
Chain gamma 3
33
8
8
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
67
17
3
Host
Rabbit
33
25
12
6
4
3
2
1
1
Clonality
Polyclonal
Conjugate
DyLight 549
10
9
9
6
5
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogen

Immunogen: Mouse IgG3 heavy chain

Isotype IgG
Characteristics

Synonyms: Rabbit Anti-Mouse IgG3 (Gamma 3 chain) DyLight 549™ Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody DyLight 549™ Conjugation

Background: Anti-Mouse IgG3 DyLight 549 Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification Preadsorption: Solid phase absorption
Labeling Ratio 3.1
Antigen
Application Notes

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiry Date 12 months
Product cited in: Baumann, Bakkers, Buettner, Hartmann, Grove, Langereis, de Groot, Mühlenhoff: "9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate." in: Nature communications, Vol. 6, pp. 7673, 2016 (PubMed).