Rabbit anti-Mouse IgG3 (Heavy Chain) Antibody (DyLight 680)
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- Target See all IgG3 products
- IgG3
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Binding Specificity
- Heavy Chain
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- DyLight 680
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Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-444-043
- Supplier
- Rockland
- Purpose
- Mouse IgG3 (Gamma 3 chain) Antibody DyLight™ 680 Conjugated
- Characteristics
- Rabbit Anti-Mouse IgG3 (Gamma 3 chain) DyLight 680™ Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody DyLight 680™ Conjugation,Anti-Mouse IgG3 DyLight 680 Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain).
- Immunogen
- Mouse IgG3 heavy chain
- Isotype
- IgG
- Labeling Ratio
- 2.4
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- Application Notes
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- Comment
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The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgG3
- Abstract
- IgG3 Products
- Target Type
- Antibody
- Background
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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