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Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 680) - Preadsorbed Secondary Antibody

FLISA, FM, WB Polyclonal IgG DyLight 680
Pubmed (6)
Catalog No. ABIN6699112
$95.00
Plus shipping costs $45.00
100 μg ABIN6699112
100 μg ABIN6699112
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  • Target
    IgG
    Binding Specificity
    • 1528
    • 134
    • 117
    • 46
    • 34
    • 30
    • 25
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 367
    • 333
    • 314
    • 224
    • 213
    • 111
    • 110
    • 82
    • 76
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 821
    • 781
    • 312
    • 178
    • 76
    • 75
    • 22
    • 20
    • 16
    • 12
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Goat
    Clonality
    • 1786
    • 177
    Polyclonal
    Conjugate
    • 317
    • 233
    • 216
    • 207
    • 147
    • 146
    • 128
    • 72
    • 49
    • 46
    • 36
    • 31
    • 29
    • 28
    • 26
    • 26
    • 26
    • 25
    • 25
    • 24
    • 24
    • 24
    • 23
    • 23
    • 23
    • 23
    • 23
    • 22
    • 21
    • 19
    • 19
    • 18
    • 18
    • 14
    • 12
    • 11
    • 10
    • 9
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    DyLight 680
    Application
    • 1261
    • 1111
    • 917
    • 806
    • 539
    • 524
    • 498
    • 144
    • 100
    • 71
    • 71
    • 65
    • 52
    • 50
    • 29
    • 28
    • 16
    • 13
    • 11
    • 10
    • 10
    • 9
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
    Purpose
    Goat anti-Rabbit IgG DyLight™ 680 Conjugated Antibody
    Specificity
    This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.
    Characteristics

    Synonyms: Goat anti-Rabbit IgG Antibody DyLight™680 Conjugation, Goat anti-Rabbit IgG DyLight™ 680 Conjugated Antibody

    Background: Anti-Rabbit IgG Antibody DyLight™680 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™680.

    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
    Immunogen
    Rabbit IgG whole molecule
    Isotype
    IgG
    Labeling Ratio
    2.6
  • Application Notes

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Xia, Ji, Xu, Lin, Wang, Xia, Lv, Song, Ma, Chen: "Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress." in: Cell death & disease, Vol. 8, Issue 7, pp. e2957, 2018 (PubMed).

    Li, Xu, Lin, Qu, Xia, Hongdu, Xia, Wang, Lou, He, Ma, Chen: "Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality." in: Cell death & disease, Vol. 8, Issue 5, pp. e2811, 2018 (PubMed).

    Lin, Cui, Xu, Hong, Xia, Xu, Li, Zhang, Lou, He, Lv, Chen: "Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy." in: Cell death & disease, Vol. 9, Issue 7, pp. 768, 2018 (PubMed).

    Shen, Kan, Hu, Li, Lu, Zhang, Zhang, Hou, Chen, Bai: "EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy." in: Cell death & disease, Vol. 7, pp. e2043, 2016 (PubMed).

    Wang, Hu, Li, Qu, He, Lou, Song, Ma, Chen: "PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1." in: Autophagy, Vol. 10, Issue 12, pp. 2158-70, 2015 (PubMed).

    Li, Zhao, Hu, Xiao, Qu, Wang, Ma, Chen: "A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy." in: Autophagy, Vol. 9, Issue 2, pp. 150-63, 2013 (PubMed).

  • Target
    IgG
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