Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 800) - Preadsorbed Secondary Antibody
- Heavy & Light Chain
- DyLight 800
- FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
- Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody
- This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.
Synonyms: Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody
Background: Anti-Rabbit IgG Antibody DyLight™800 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™800.
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
- Rabbit IgG whole molecule
- Labeling Ratio
- Application Notes
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- For Research Use only
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
Cao, Xue, Cheng, Wang, Liu, Li, Jiang, Li, Gui, Zhang: "MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1." in: Oncogene, Vol. 38, Issue 24, pp. 4835-4855, 2019 (PubMed).
Heubl, Zhang, Pressey, Al Awabdh, Renner, Gomez-Castro, Moutkine, Eugène, Russeau, Kahle, Poncer, Lévi: "GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl--sensitive WNK1 kinase." in: Nature communications, Vol. 8, Issue 1, pp. 1776, 2018 (PubMed).
Xia, Ji, Xu, Lin, Wang, Xia, Lv, Song, Ma, Chen: "Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress." in: Cell death & disease, Vol. 8, Issue 7, pp. e2957, 2018 (PubMed).
Li, Xu, Lin, Qu, Xia, Hongdu, Xia, Wang, Lou, He, Ma, Chen: "Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality." in: Cell death & disease, Vol. 8, Issue 5, pp. e2811, 2018 (PubMed).
Lin, Cui, Xu, Hong, Xia, Xu, Li, Zhang, Lou, He, Lv, Chen: "Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy." in: Cell death & disease, Vol. 9, Issue 7, pp. 768, 2018 (PubMed).
Zhang, Kang, Zhou, Cui, Jia, Hu, Ji, Yuan, Cui, Shi: "Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling." in: Scientific reports, Vol. 8, Issue 1, pp. 10726, 2018 (PubMed).
Myles, Anderson, Earland, Zarember, Sastalla, Williams, Gough, Moore, Ganesan, Fowler, Laurence, Garofalo, Kuhns, Kieh, Saleem, Welch, Darnell, Gallin, Freeman, Holland, Datta: "TNF overproduction impairs epithelial staphylococcal response in hyper IgE syndrome." in: The Journal of clinical investigation, Vol. 128, Issue 8, pp. 3595-3604, 2018 (PubMed).
Wang, Cao, Xue, Li, Jiang, Cheng, Li, Zhang: "A positive feedback loop between Pim-1 kinase and HBP1 transcription factor contributes to hydrogen peroxide-induced premature senescence and apoptosis." in: The Journal of biological chemistry, Vol. 292, Issue 20, pp. 8207-8222, 2017 (PubMed).
Wang, Gregory-Evans, Wasan, Sivak, Shan, Gregory-Evans: "Efficacy of Postnatal In Vivo Nonsense Suppression Therapy in a Pax6 Mouse Model of Aniridia." in: Molecular therapy. Nucleic acids, Vol. 7, pp. 417-428, 2017 (PubMed).
Chen, Pan, Wang, Cao, Wang, Wang, Hu, Xue, Li, Jiang, Li, Zhang: "HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis." in: The Journal of biological chemistry, Vol. 291, Issue 24, pp. 12688-705, 2016 (PubMed).
Shen, Kan, Hu, Li, Lu, Zhang, Zhang, Hou, Chen, Bai: "EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy." in: Cell death & disease, Vol. 7, pp. e2043, 2016 (PubMed).
Wang, Hu, Li, Qu, He, Lou, Song, Ma, Chen: "PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1." in: Autophagy, Vol. 10, Issue 12, pp. 2158-70, 2015 (PubMed).
Wang, Chen, Wang, Hu, Cao, Wang, Tong, Zhang: "PIASxα ligase enhances SUMO1 modification of PTEN protein as a SUMO E3 ligase." in: The Journal of biological chemistry, Vol. 289, Issue 6, pp. 3217-30, 2014 (PubMed).
Li, Zhao, Hu, Xiao, Qu, Wang, Ma, Chen: "A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy." in: Autophagy, Vol. 9, Issue 2, pp. 150-63, 2013 (PubMed).
- Cao, Xue, Cheng, Wang, Liu, Li, Jiang, Li, Gui, Zhang: "MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1." in: Oncogene, Vol. 38, Issue 24, pp. 4835-4855, 2019 (PubMed).