Nuclear Pore Complex antibody

Catalog No. ABIN6699198

Product Details

Target: Nuclear Pore Complex

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Nuclear Pore Complex antibody is directed against Nuclear Pore Complex proteins. The antibody is protein G purified from concentrated clarified cell culture supernate. This antibody is directed against yeast NPC protein. Reactivity is expected from human, mouse and rat.

Immunogen:

Immunogen: Anti-Nuclear Pore Complex Antibody (Monoclonal) was produced by repeated immunizations with yeast nuclear preparations.

Immunogen Type: Native Protein

Clone: 39C7

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Nuclear Pore Complex Antibody is suitable for use in IF. Specific conditions for reactivity should be optimized by the end user

IF Microscopy Dilution: 1:50-100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details

Target: Nuclear Pore Complex

Background:

Synonyms: mouse anti-Nuclear Pore Complex Antibody, NPC, NUP62, NUP, NUP133

Background: Nuclear Pore Complex Antibodydetects Nuclear Pore Complex proteins. The nuclear pore complex (NPC) is a very large structure made up of at least 50 different proteins that span the double membrane of the nuclear envelope functioning as a gateway for macromolecular traffic between the cytoplasm and the nucleus. Discrete nuclear pore complex proteins or nucleoporins such as NUP98, NUP180 and p62 have been implicated in autoimmune disease and cancer. Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against p62 and NUP180 while NUP98 translocations have been found in patients with acute myelogenous leukemia (AML). Anti-Nuclear Pore Complex Antibody is ideal for investigators involved in Cancer and Immunology Research.

Gene Name: Nuclear stain of multiple gene products including Nup62, Nup133