TUBB3 antibody (Tubulin, Beta, 3)

Details for Product anti-TUBB3 Antibody No. ABIN6699201
Antigen
  • CDCBM
  • CFEOM3A
  • TUBB4
  • beta-4
  • TUBB3
  • 3200002H15Rik
  • M(beta)3
  • M(beta)6
  • tubb3
  • tubulin beta 3 class III
  • tubulin, beta 3 class III
  • tubulin beta 3 class III L homeolog
  • beta-tubulin 3
  • tubulin beta-3 chain
  • beta tubulin 3
  • TUBB3
  • Tubb3
  • tubb3
  • tubb3.L
  • LOC100581245
  • tub3
Reactivity
Rat (Rattus)
290
155
147
15
11
8
7
6
6
5
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
171
128
17
7
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBB3 antibody is un-conjugated
24
23
13
9
9
9
9
9
9
3
3
3
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
247
128
89
53
50
45
35
17
16
16
13
9
8
6
4
Options
Immunogen

Immunogen: Anti-Tubulin beta 3 Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues specific to beta III tubulin.

Immunogen Type: Peptide
Clone AA10
Isotype IgG
Cross-Reactivity (Details) Cross reactivity with tubulin beta 3 from other species has not been determined
Purification Anti-Tubulin beta 3 Antibody is directed against rat Tubulin beta III protein. The antibody was Protein G purified. .
Plasmids, Primers & others Plasmids, Primers & others TUBB3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Tubulin beta 3 (TUBB3 Antibody Abstract)
Background

Synonyms: mouse anti-Tubulin beta 3 Antibody, Neuron-specific class III beta-tubulin, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

Background: Tubulin beta 3 Antibody detects Tubulin beta III protein. Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins. Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy.

Gene Name: TUBB3
Gene ID 246118
UniProt Q4QRB4
Pathways Microtubule Dynamics, M Phase
Application Notes

Immunohistochemistry Dilution: 1:1,000

Application Note: Anti-Tubulin beta 3 (Mouse) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to beta III tubulin proteins in the appropriate cell lysate or extract.

Western Blot Dilution: 1:10,000
Restrictions For Research Use only
Format Liquid
Buffer

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol
Storage 4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Supplier Images
Image no. 1 for anti-Tubulin, Beta, 3 (TUBB3) antibody (ABIN6699201) Immunohistochemistry - Mouse Anti-Tubulin B III antibody Immunohistochemistry of Mous...
Image no. 2 for anti-Tubulin, Beta, 3 (TUBB3) antibody (ABIN6699201) Western Blot - Mouse Anti-Tubulin B III antibody Western Blot of Mouse Anti-Tubulin ß...
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