TUBB3 antibody

Catalog No. ABIN6699201

Supplier Product No.: 212-301-e55

Product Details anti-TUBB3 Antibody

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 212-301-e55

Supplier: Rockland

Purpose: Tubulin beta 3 Antibody

Cross-Reactivity (Details): Anti-Tubulin beta 3 Antibody is directed against rat Tubulin beta III protein.

Purification: The antibody was Protein G purified from cell culture supernatant.

Immunogen: Anti-Tubulin beta 3 Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues specific to beta III tubulin.

Clone: AA10

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1,000

Western_Blot_Dilution: 1:10,000

Other: User Optimized

Comment:

Anti-Tubulin beta 3 (Mouse) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to beta III tubulin proteins in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: Tubulin beta 3 (TUBB3 Products)

Background:

Synonyms: mouse anti-Tubulin beta 3 Antibody, Neuron-specific class III beta-tubulin, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

Background: Tubulin beta 3 Antibody detects Tubulin beta III protein. Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins. Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy.

Gene Name: TUBB3

Gene ID: 246118

UniProt: Q4QRB4

Pathways: Microtubule Dynamics, M Phase