TUBB3 antibody

Catalog No. ABIN6699201

Product Details anti-TUBB3 Antibody

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity with tubulin beta 3 from other species has not been determined

Purification: Anti-Tubulin beta 3 Antibody is directed against rat Tubulin beta III protein. The antibody was Protein G purified. .

Immunogen:

Immunogen: Anti-Tubulin beta 3 Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues specific to beta III tubulin.

Immunogen Type: Peptide

Clone: AA10

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000

Application Note: Anti-Tubulin beta 3 (Mouse) antibody is suitable for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 55 kDa in size corresponding to beta III tubulin proteins in the appropriate cell lysate or extract.

Western Blot Dilution: 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: Tubulin beta 3 (TUBB3 Products)

Synonyms: CDCBM antibody, CFEOM3A antibody, TUBB4 antibody, beta-4 antibody, TUBB3 antibody, 3200002H15Rik antibody, M(beta)3 antibody, M(beta)6 antibody, tubb3 antibody, tubulin beta 3 class III antibody, tubulin, beta 3 class III antibody, tubulin beta 3 class III L homeolog antibody, beta-tubulin 3 antibody, tubulin beta-3 chain antibody, beta tubulin 3 antibody, TUBB3 antibody, Tubb3 antibody, tubb3 antibody, tubb3.L antibody, LOC100581245 antibody, tub3 antibody

Background:

Synonyms: mouse anti-Tubulin beta 3 Antibody, Neuron-specific class III beta-tubulin, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III

Background: Tubulin beta 3 Antibody detects Tubulin beta III protein. Tubulin is the major constituent of microtubules, existing as a heterodimer of the α and β subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins. Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy.

Gene Name: TUBB3

Gene ID: 246118

UniProt: Q4QRB4

Pathways: Microtubule Dynamics, M Phase