DYKDDDDK Tag antibody (DyLight 488)

Catalog No. ABIN6720683

Supplier Product No.: 600-441-383

Product Details anti-DYKDDDDK Tag Antibody (DyLight 488)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to DyLight 488

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA

Supplier Product No.: 600-441-383

Supplier: Rockland

Purpose: DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 488 Conjugated

Cross-Reactivity (Details): The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Purification: This affinity purified antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays.

Immunogen: This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide (D-Y-K-D-D-D-D-K) conjugated to KLH using maleimide.  This antibody reacts with FLAG™ conjugated proteins.

Isotype: IgG

Labeling Ratio: 4.0

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

ELISA_Dilution: 1:10,000 - 1:25,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Comment:

Suggested Applications: EM
Anti-FLAG DyLight 488 Conjugated Antibody has been tested by Western Blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-DYKDDDDK Tag antibody (DyLight 488) (ABIN6720683)

Mark, Lai, Zarin, Manning, Pollington, Litwin-Kumar, Cardona, Truman, Doe: "A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS." in: eLife, Vol. 10, (2021) (PubMed).

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Abstract: DYKDDDDK Tag Products

Background:

Synonyms: rabbit anti DYKDDDDK DyLight™ 488 Conjugated, rabbit antibody for the detection of FLAG™ conjugated proteins DyLight™ 488 Conjugated

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.