COL2 antibody (AA 1201-1300)

Catalog No. ABIN672171

Product Details anti-COL2 Antibody

Target: COL2 (Collagen, Type II (COL2))

Binding Specificity: AA 1201-1300

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Guinea Pig, Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Collagen II

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-COL2 antibody (ABIN672171)

Chen, Kuo, Wang, Chen: "Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering." in: International journal of molecular sciences, Vol. 17, Issue 11, (2017) (PubMed).

Liu, Yu, Ma, Xiang, Hu, Chen: "Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model." in: Experimental and therapeutic medicine, Vol. 11, Issue 4, pp. 1399-1404, (2016) (PubMed).

Fu, Liao, Lin, Sun, Tian, Zhu, Lin: "PCL-PEG-PCL film promotes cartilage regeneration in vivo." in: Cell proliferation, Vol. 49, Issue 6, pp. 729-739, (2016) (PubMed).

Wang, Yang, Lin, Liu, Yang, Kuo, Chen: "Expandable Scaffold Improves Integration of Tissue-Engineered Cartilage: An In Vivo Study in a Rabbit Model." in: Tissue engineering. Part A, Vol. 22, Issue 11-12, pp. 873-84, (2016) (PubMed).

Li, Niu, Wu, Du, Huang, Yin, Liu, Song, Leng: "Vitamin D prevents articular cartilage erosion by regulating collagen II turnover through TGF-β1 in ovariectomized rats." in: Osteoarthritis and cartilage, Vol. 24, Issue 2, pp. 345-53, (2016) (PubMed).

Jeong, Yang, Lee, Ryoo, Kim, Oh, Kwon, Liu, Son: "Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation." in: Stem cell research & therapy, Vol. 7, Issue 1, pp. 117, (2016) (PubMed).

Wu, Zhu, Sun, Hu: "Subchondral bone changes and chondrogenic capacity of progenitor cells from subchondral bone in the collagenase-induced temporomandibular joints osteoarthritis rabbit model." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 9782-9, (2016) (PubMed).

Chen, Chen, Jhan, Liu, Ho, Mao, Kohn, Sheu: "Development and Characterization of Acellular Extracellular Matrix Scaffolds from Porcine Menisci for Use in Cartilage Tissue Engineering." in: Tissue engineering. Part C, Methods, (2015) (PubMed).

Li, Fu, Xie, Fu, Deng, Cun, Wei, Peng, Cai, Lin: "Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Based Electrospun 3D Scaffolds for Delivery of Autogeneic Chondrocytes and Adipose-Derived Stem Cells: Evaluation of Cartilage Defects in Rabbit." in: Journal of biomedical nanotechnology, Vol. 11, Issue 1, pp. 105-16, (2015) (PubMed).

Jiang, Tian, Wang, Yan, Liu, Liu, Song, Zhang, Shen, Zhang: "Effect of calcitonin pretreatment on naturally occurring intervertebral disc degeneration in guinea pig." in: International journal of clinical and experimental medicine, Vol. 8, Issue 7, pp. 10367-79, (2015) (PubMed).

Zheng, Sun, Li, Zhou, Fan, Zhang: "Comparative study of collagen hydrogels modified in two ways using the model of ectopic cartilage construction with diffusion-chamber in immunocompetent host." in: Journal of applied biomaterials & functional materials, Vol. 12, Issue 1, pp. 41-7, (2014) (PubMed).

Kuo, Chen, Hsiao, Chen: "Incorporation of chitosan in biomimetic gelatin/chondroitin-6-sulfate/hyaluronan cryogel for cartilage tissue engineering." in: Carbohydrate polymers, Vol. 117, pp. 722-30, (2014) (PubMed).

Xu, Dai, Liu, Liu, Song, Li, Chen, Lin: "Sanmiao formula inhibits chondrocyte apoptosis and cartilage matrix degradation in a rat model of osteoarthritis." in: Experimental and therapeutic medicine, Vol. 8, Issue 4, pp. 1065-1074, (2014) (PubMed).

Luo, Zhang, Wang, Hu, Song, Su, Zhang: "Alendronate retards the progression of lumbar intervertebral disc degeneration in ovariectomized rats." in: Bone, Vol. 55, Issue 2, pp. 439-48, (2013) (PubMed).

Wang, Bian, Wu, Chen: "Evaluation of three-dimensional scaffolds prepared from poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) for growth of allogeneic chondrocytes for cartilage repair in rabbits." in: Biomaterials, Vol. 29, Issue 19, pp. 2858-68, (2008) (PubMed).

Target Details for COL2

Target: COL2 (Collagen, Type II (COL2))

Alternative Name: Collagen 2 (COL2 Products)

Background:

Synonyms: AOM, ANFH, SEDC, STL1, COL11A3, Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

Background: Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Molecular Weight: 156kDa

Gene ID: 1280

UniProt: P02458