GNB2L1 antibody (C-Term)

Catalog No. ABIN6736725

Product Details anti-GNB2L1 Antibody

Target: GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Sheep, Zebrafish (Danio rerio), Chicken, Xenopus laevis, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNB2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GNB2L1 / RACK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human GNB2L1 (P63244, NP_006089). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Trout, Catfish, Salmon, Smelt, Sablefish, Stickleback, Pike, Pufferfish, Zebrafish, Penicillium (100%), Sea squirt, Nematode (92%), Drosophila, Beetle (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for GNB2L1

Target: GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))

Alternative Name: GNB2L1 / RACK1 (GNB2L1 Products)

Background: Name/Gene ID: RACK1

Synonyms: RACK1, H12.3, Gnb2-rs1, Proliferation-inducing gene 21, GNB2L1, HLC-7, Lung cancer oncogene 7, PIG21

Gene ID: 10399

NCBI Accession: NP_006089

UniProt: P63244

Pathways: cAMP Metabolic Process, Positive Regulation of Endopeptidase Activity