Hemagglutinin antibody (AA 200-300)

Catalog No. ABIN679238

The Rabbit Polyclonal anti-Hemagglutinin antibody has been validated for ELISA, WB, IF (p), IF (cc), IHC (p) and IHC (fro). It is suitable to detect Hemagglutinin in samples from Influenza A Virus H5N1 and Virus. There are 2+ publications available.

Quick Overview for Hemagglutinin antibody (AA 200-300) (ABIN679238)

Target: Hemagglutinin (HA)

Reactivity: Influenza A Virus H5N1, Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hemagglutinin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Hemagglutinin Antibody

Binding Specificity: AA 200-300

Virus Strain: A/chicken/West Bengal/100879/2008

Cross-Reactivity: Virus

Cross-Reactivity (Details): Influenza A virus H5N1

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Hemagglutinin/Influenza A virus (H5N1)

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Hemagglutinin antibody (ABIN679238)

Lv, Li, Liu, Li, Liu, Shen, Wang, Xue, Cao: "Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus." in: Journal of veterinary science, Vol. 15, Issue 2, pp. 209-16, (2015) (PubMed).

Lee, Na, Yang, Choi, Seo, Hong, Yun, Kim, Sohn, Kim, Sung, Kim, Jang, Hwang: "Oral immunization of haemaggulutinin H5 expressed in plant endoplasmic reticulum with adjuvant saponin protects mice against highly pathogenic avian influenza A virus infection." in: Plant biotechnology journal, (2014) (PubMed).

Target Details for Hemagglutinin

Target: Hemagglutinin (HA)

Alternative Name: H5N1 Hemagglutinin

Target Type: Influenza Protein

Background:

Synonyms: [Influenza A virus A/chicken/West Bengal/100879/2008H5N1], Influenza A virus Hemagglutinin[H5N1.

Background: Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals, however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong. There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species jumping ability. Influenza A Virus Hemagglutinin recognize the influenza hemagglutinin epitope, which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of this antibody allows for unambiguous identification and quantitative analysis of the tagged protein.