Hemagglutinin antibody (AA 151-250)

Catalog No. ABIN676538

The Rabbit Polyclonal anti-Hemagglutinin antibody has been validated for ELISA, WB, IF (p), IF (cc), IHC (p) and IHC (fro). It is suitable to detect Hemagglutinin in samples from Influenza A Virus H1N1 and Virus.

Quick Overview for Hemagglutinin antibody (AA 151-250) (ABIN676538)

Target: Hemagglutinin (HA)

Reactivity: Influenza A Virus H1N1, Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hemagglutinin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Hemagglutinin Antibody

Binding Specificity: AA 151-250

Virus Strain: A/California/04/2009

Cross-Reactivity: Virus

Cross-Reactivity (Details): Influenza A virus H1N1 (strain swl A/California/04/2009 H1N1)

Characteristics: Antibody is specific for: Influenza A virus H1N1 (strain swl A/California/04/2009 H1N1)

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Influenza A Virus Hemagglutinin (strain swl A/California/04/2009 H1N1)

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Hemagglutinin

Target: Hemagglutinin (HA)

Target Type: Influenza Protein

Background:

Synonyms: HA, Hemagglutinin, Influenza A Virus (strain swl A/California/04/2009 H1N1)

Background: Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals, however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Influenza A Virus (strain swl A/California/04/2009 H1N1)

UniProt: C3W5S1