FTH1 antibody (AA 58-180)

Catalog No. ABIN6937271

Product Details anti-FTH1 Antibody

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Binding Specificity: AA 58-180

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of aging-related cataracts.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary)

Clone: FTH-2081

Isotype: IgG2a kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Alternative Name: FTH1 (FTH1 Products)

Background: Apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, Ferritin heavy chain, FHC, FRIH, FTH1, FTHL6, Iron overload autosomal dominant, PIG15, Placenta immunoregulatory factor, Proliferation inducing gene 15 protein (PLIF),Ferritin, Heavy Chain (FTH) (Microglia Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 21kDa

Gene ID: 2495, 524910, 645560

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis