FCGR2A antibody

Catalog No. ABIN6939375

Product Details anti-FCGR2A Antibody

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCGR2A antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Functional Studies (Func)

Specificity: This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Purification: Purified by Protein A/G

Immunogen: K562 and FcgRII+L cells

Clone: 7-3

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: K-562 cells. Lymph node, tonsil and placenta.

Known Application: Functional Studies (Order Ab without BSA & Azide)Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FCGR2A

Target: FCGR2A (Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))

Alternative Name: FCGR2A (FCGR2A Products)

Molecular Weight: 40kDa

Gene ID: 2212

UniProt: P12318