Oct-2 antibody (AA 112-297)

Catalog No. ABIN6940358

Product Details anti-Oct-2 Antibody

Target: Oct-2 (POU2F2) (POU domain, class 2, transcription factor 2 (POU2F2))

Binding Specificity: AA 112-297

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Oct-2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human OCT2 protein (around aa 112-297) (exact sequence is proprietary)

Clone: Oct2-2136

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: Raji or U-937 cells. Spleen or Tonsil.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Oct-2

Target: Oct-2 (POU2F2) (POU domain, class 2, transcription factor 2 (POU2F2))

Alternative Name: POU2F2 (POU2F2 Products)

Background: Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. Oct-2 expression can be used as a marker of B-cell lineage and differentiation. Germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells show high level expression of Oct-2. Additionally, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, Hodgkin lymphoma display increased expression of Oct-2. Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines have high levels of expression. In spite of scanty evidence for Oct-2 expression in T cells, it is believed that this factor participates in transcriptional regulation during T-cell activation.

Molecular Weight: 60kDa

Gene ID: 5452

UniProt: P09086