VISTA antibody

Catalog No. ABIN6940575

Product Details anti-VISTA Antibody

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VISTA antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogen: Recombinant full-length human VISTA protein

Clone: VISTA-3007

Isotype: IgG2b kappa

Application Details

Application Notes:

Positive Control: Spleen or Placenta.

Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for VISTA

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Alternative Name: VSIR (VISTA Products)

Background: VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.

Molecular Weight: 34kDa

Gene ID: 64115

UniProt: Q9H7M9

Pathways: Cancer Immune Checkpoints