CD40 antibody (CD40 Molecule, TNF Receptor Superfamily Member 5) Primary Antibody
This CD40 antibody is un-conjugated
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
- Purified by Protein A/G
- Recombinant full-length human CD40 protein
- IgG1 kappa
- Application Notes
Positive Control: U2OS, L-428 cells. Tonsil, Spleen or Thymus.
Known Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- For Research Use only
- 200 μg/mL
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
- Alternative Name
- CD40 (CD40 Antibody Abstract)
- Bp50, CDW40, TNFRSF5, p50, AI326936, GP39, HIGM1, IGM, IMD3, T-BAM, TRAP, Tnfrsf5, TNFSF5, CD40 molecule, CD40 antigen, CD40, Cd40
- CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.
- Molecular Weight
- Gene ID
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints