Human Papilloma Virus 16 E2 (HPV-16 E2) antibody

Catalog No. ABIN6941308

Product Details

Target: Human Papilloma Virus 16 E2 (HPV-16 E2)

Reactivity: Human Papillomavirus 16 (HPV-16)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Purification: Purified by Protein A/G

Immunogen: Raised against Vaccinia-E2 followed by intravenous injection of the maltose binding protein MBP-E2

Clone: TVG 261

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HPV-16 infected cells. Cervical tissue.

Known Application: Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Human Papilloma Virus 16 E2 (HPV-16 E2)

Alternative Name: HPV16 E2 (Human Papilloma Virus 16)

Target Type: Viral Protein

Background: The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a strain that is responsible for the largest number of cases of cervical cancers linked to the family. HPV16E1 and HPV16E2 are proteins that are involved in the regulation of viral DNA replication and are important for infected cell homeostasis. HPV16E2 specifically regulates the expression of the E6 and E7 oncoproteins by binding to four sites within the viral long control region, possibly involving interactions with nuclear hormone receptors. Integration of the HPV genome into the host DNA usually disrupts the HPV16E2 gene open reading frames, resulting in an overexpression of E6 and E7 genes, an event that may lead to the malignant transformation of cervical cancer. HPV16E2 is also able to induce apoptotic cell death via two pathways: the first through the binding of p53 and the second through the binding of the viral genome.

Molecular Weight: 43kDa