POLR2A antibody (Polymerase (RNA) II (DNA Directed) Polypeptide A, 220kDa) (pSer5)

Details for Product anti-POLR2A Antibody No. ABIN6944639
Antigen
  • NCU02103.1
  • 19.m02399
  • POLR2A
  • 220kDa
  • Rpb1
  • Rpo2-1
  • POLR2
  • POLRA
  • RPB1
  • RPBh1
  • RPO2
  • RPOL2
  • RpIILS
  • hRPB220
  • hsRPB1
  • DNA-directed RNA polymerase II largest subunit
  • DNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II large subunit Rpb1
  • RNA polymerase II subunit A
  • DNA-directed RNA polymerase II largest subunit, putative
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
  • polymerase (RNA) II (DNA directed) polypeptide A
  • NCU02103
  • BBOV_I003360
  • EDI_169920
  • CMU_000800
  • SJAG_01713
  • MGYG_01527
  • POLR2A
  • TP03_0185
  • EBI_22514
  • CGB_E5020W
  • polr2a
  • Polr2a
Epitope
pSer5
21
17
16
9
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
121
80
71
8
8
8
8
7
5
4
4
3
2
2
2
1
1
1
Host
Rabbit
98
23
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This POLR2A antibody is un-conjugated
5
5
5
5
5
5
5
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
81
41
40
35
18
9
7
7
5
4
4
4
3
3
2
1
1
Options
Immunogen Synthetic phospho-peptide corresponding to residues surrounding Ser5 of human POLR2A
Clone 12C8
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others POLR2A products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name POLR2A (POLR2A Antibody Abstract)
Background

RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.


Synonyms: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB2, RNA polymerase II subunit B1, POLR3A
Gene ID 5430
UniProt P24928
Pathways Regulatory RNA Pathways
Application Notes WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200)
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months
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