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POLR2A/RPB1 antibody (pSer5)

POLR2A Reactivity: Human WB, ELISA, ChIP, FM, CUT&RUN Host: Mouse Monoclonal unconjugated
Catalog No. ABIN6655367
  • Target See all POLR2A/RPB1 (POLR2A) Antibodies
    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
    Binding Specificity
    • 17
    • 16
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer5
    Reactivity
    • 89
    • 47
    • 39
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 73
    • 16
    • 1
    • 1
    • 1
    Mouse
    Clonality
    • 55
    • 37
    Monoclonal
    Conjugate
    • 54
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    • 1
    This POLR2A/RPB1 antibody is un-conjugated
    Application
    • 49
    • 26
    • 22
    • 20
    • 13
    • 11
    • 8
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    Purification
    Anti-Pol II pS5 Antibody was purified by Protein A chromatography. This antibody is specific for Pol II protein phosphorylated Ser5. Cross-reactivity with Pol II pS5 from other sources has not been determined.
    Immunogen

    Immunogen: Anti-Pol II S5p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser5 of the repeated sequence.

    Immunogen Type: Peptide

    Isotype
    IgG1
    Top Product
    Discover our top product POLR2A Primary Antibody
  • Application Notes
    The rabbit anti-PolII (pSer5) antibody ABIN6655367 is suitable for use in ChIP, CUT&RUN, ELISA, immunofluorescence microscopy, and western blot. Specific conditions for each assay should be optimized by the end user. Expect a band of approximately 225 kDa in western blot in the appropriate cell lysate or extract. General ABIN6655367 dilution recommendations for different applications are as follows:
    • ChIP: 1 µg/ChIP
    • ELISA: 1:3,000
    • IF: 1:500
    • WB: 1:1,000
    • CUT&RUN: 1:100
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

    0.02 % (w/v) Sodium Azide

    Stabilizer: None

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
    Alternative Name
    Pol II (POLR2A Products)
    Synonyms
    NCU02103.1 antibody, 19.m02399 antibody, POLR2A antibody, 220kDa antibody, Rpb1 antibody, Rpo2-1 antibody, POLR2 antibody, POLRA antibody, RPB1 antibody, RPBh1 antibody, RPO2 antibody, RPOL2 antibody, RpIILS antibody, hRPB220 antibody, hsRPB1 antibody, DNA-directed RNA polymerase II largest subunit antibody, DNA-directed RNA polymerase II subunit RPB1 antibody, RNA polymerase II large subunit Rpb1 antibody, RNA polymerase II subunit A antibody, DNA-directed RNA polymerase II largest subunit, putative antibody, polymerase (RNA) II (DNA directed) polypeptide A, 220kDa antibody, polymerase (RNA) II (DNA directed) polypeptide A antibody, NCU02103 antibody, BBOV_I003360 antibody, EDI_169920 antibody, CMU_000800 antibody, SJAG_01713 antibody, MGYG_01527 antibody, POLR2A antibody, TP03_0185 antibody, EBI_22514 antibody, CGB_E5020W antibody, polr2a antibody, Polr2a antibody
    Background

    Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

    Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S5p Antibody is ideal for research in Gene Expression, Transcription and Genetics.

    Gene Name: POLR2A

    Gene ID
    5430
    NCBI Accession
    NP_000928
    UniProt
    P24928
    Pathways
    Regulatory RNA Pathways
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