PP2A alpha/beta antibody

Catalog No. ABIN6944644

Product Details anti-PP2A alpha/beta Antibody

Target: PP2A alpha/beta (PP2A A/B)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PP2A alpha/beta antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Purification: Purified by Protein A.

Immunogen: Human PP2A alpha + beta aa 250-350

Clone: 11F10

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for PP2A alpha/beta

Target: PP2A alpha/beta (PP2A A/B)

Alternative Name: PP2A alpha + beta (PP2A A/B Products)

Background:

Synonyms: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2A-alpha, RP-C, PPP2CA, PP2A-beta, PPP2CB

Background: The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases.

UniProt: P62714, P67775