ATG16L1 antibody (AA 190-240)

Catalog No. ABIN6990840

Product Details anti-ATG16L1 Antibody

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Binding Specificity: AA 190-240

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG16L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: ATG16 Antibody is affinity chromatography purified via peptide column.

Immunogen: ATG16 antibody was raised against a 18 amino acid synthetic peptide from near the center of human ATG16. The immunogen is located within amino acids 190 - 240 of ATG16.

Isotype: IgG

Application Details

Application Notes: ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.25 - 0.5 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2 μ,g/mL. For immunofluorescence start at 4 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ATG16 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ATG16 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATG16L1

Target: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Alternative Name: ATG16 (ATG16L1 Products)

Background: ATG16 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

Gene ID: 55054

NCBI Accession: NP_110430

UniProt: Q676U5

Pathways: Autophagy