SEMA3A antibody (AA 580-664)

Catalog No. ABIN7428446

Product Details anti-SEMA3A Antibody

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Binding Specificity: AA 580-664

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SEMA3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Semaphorin 3A (SEMA3A)

Specificity: The antibody is a mouse monoclonal antibody raised against SEMA3A. It has been selected for its ability to recognize SEMA3A in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Semaphorin 3A (SEMA3A) corresdonding to Pro580~Thr664 with N-terminal His Tag

Clone: C2

Isotype: IgG2a kappa

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SEMA3A

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Alternative Name: Semaphorin 3A (SEMA3A Products)

Background: SEMAL, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SemD, Coll-1, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

Pathways: Regulation of Cell Size