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Minichromosome Maintenance Deficient 5 (AA 331-537) antibody

Reactivity: Human IHC, IP, WB, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7435823
  • Target
    Minichromosome Maintenance Deficient 5
    Binding Specificity
    AA 331-537
    Reactivity
    Human
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    Un-conjugated
    Application
    Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Minichromosome Maintenance Deficient 5 (MCM5)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against MCM5. It has been selected for its ability to recognize MCM5 in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Mouse, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Minichromosome Maintenance Deficient 5 (MCM5) corresdonding to Val331~Ile537 (Accession # P33992)
    Isotype
    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:250-1000 Immunohistochemistry: 5-20 μg/mL

    1:25-100 Immunocytochemistry: 5-20 μg/mL

    1:25-100 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Minichromosome Maintenance Deficient 5
    Background
    CDC46, P1-CDC46, Cell Division Cycle 46, DNA replication licensing factor MCM5
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