TAF5 antibody

Catalog No. ABIN7263209

Product Details anti-TAF5 Antibody

Target: TAF5 (TAF5 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 100kDa (TAF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human TAF5 (NP_008882.2).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IF 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for TAF5

Target: TAF5 (TAF5 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 100kDa (TAF5))

Alternative Name: TAF5 (TAF5 Products)

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants.

Molecular Weight:

Observed_MW: 100 kDa

Calculated_MW: 80 kDa/86 kDa

Gene ID: 6877

UniProt: Q15542