Adenosine A2b Receptor antibody (2nd Extracellular Loop, Cys154)

Catalog No. ABIN7042889

Product Details anti-Adenosine A2b Receptor Antibody

Target: Adenosine A2b Receptor (ADORA2B)

Binding Specificity: 2nd Extracellular Loop, AA 147-166, Cys154

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A2b Receptor antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Live Cell Imaging (LCI), Immunocytochemistry (ICC)

Characteristics: Anti-Adenosine A2B Receptor (extracellular) Antibody (ABIN7042889, ABIN7043890 and ABIN7043891)) is a highly specific antibody directed against an extracellular epitope of human adenosine A2B receptor (A2BAR). The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize A2BAR from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR with replacement of cysteine 154 (C154) with serine (*S)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.75 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Adenosine A2b Receptor

Target: Adenosine A2b Receptor (ADORA2B)

Alternative Name: Adenosine A2B Receptor (ADORA2B Products)

Background: Alternative names: Adenosine A2B Receptor, ADORA2b, A2BAR, Adenosine A2bR

Gene ID: 136

NCBI Accession: NM_000676

UniProt: P29275

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process