Anoctamin 2 antibody (Extracellular)

Catalog No. ABIN7042928

Product Details anti-Anoctamin 2 Antibody

Target: Anoctamin 2 (ANO2)

Binding Specificity: AA 632-646, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Anoctamin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Anoctamin-2 (TMEM16B) Channel

Specificity: 3rd extracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: human - 13,Rat - 14,15 amino acid residues identical

Characteristics: Anti-TMEM16B (ANO2) (extracellular) Antibody (ABIN7042928, ABIN7044133 and ABIN7044134)) is a highly specific antibody directed against an epitope of mouse Anoctamin-2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Anoctamin-2 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HSKRPEQWDLDHSLE, corresponding to amino acid residues 632-646 of mouse Anoctamin-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-CL012

Blocking Peptide: BLP-CL012

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Anoctamin 2

Target: Anoctamin 2 (ANO2)

Alternative Name: ANO2 (ANO2 Products)

Background: Anoctamin-2, Transmembrane protein 16B, C12orf3,Anoctamin (ANO or TMEM16) is a family of 10 membrane proteins. This family is named so because these channels are selective to ANions and have eight (OCT) transmembrane domains. Also, these channels are subject to glycosylation in their extracellular loops and have both intracellular N- and C-termini1. Members of this family are expressed in a broad range of different organisms ranging from mammals, flies, worms, plants and yeast1. Alternative splicing is known to affect these channels and regarding their oligomerization state, homedimerization has been observed although when heterologously expressed, these channels may hetero oligomerize2.Ano1 (or TMEM16A, DOG1 and others) the first member to be identified was found to be a Ca2+-activated Cl- channel3-5 therefore other members are likely to also be Cl- channels. These channels are expressed in many different tissues: bronchiolar epithelial cells, pancreatic acinar cells, proximal kidney tubule epithelium, retina, dorsal root ganglia and submandibular gland1. In fact, Ano1 gained a lot of attention as its activation may serve as a therapeutic treatment for cystic fibrosis since it is also expressed in the airways6. These Ca2+-activated Cl- channels are believed to play a role in development as knockout of Ano1 in mice causes abnormal development of the trachea7. Ano2 (TMEM16B) has been shown to mediate Ca2+-activated Cl- current in olfactory epithelium and photoreceptor synapses2,8,9.Although relatively newly discovered channels, they are being discovered in many medical indications. Ano1 has become a marker in gastrointestinal tumors as its expression is significantly upregulated10,11. Similarly, Ano1 is also highly expressed in other carcinomas12,13.

Alternative names: TMEM16B (ANO2), Anoctamin-2, Transmembrane protein 16B, C12orf3

Gene ID: 243634

NCBI Accession: NM_020373

UniProt: Q8CFW1