Anoctamin 6 antibody (Extracellular)

Catalog No. ABIN7042930

Product Details anti-Anoctamin 6 Antibody

Target: Anoctamin 6 (ANO6)

Binding Specificity: AA 487-500, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Anoctamin 6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Anoctamin-6 Channel

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 12,14 amino acid residues identical

Characteristics: Anti-Anoctamin-6 (extracellular) Antibody (ABIN7042930, ABIN7044137 and ABIN7044138)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, and live cell flow cytometry applications. It has been designed to recognize ANO6 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KLPKNINGTDPIQK, corresponding to amino acid residues 487-500 of human ANO6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-CL016

Blocking Peptide: BLP-CL016

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Anoctamin 6

Target: Anoctamin 6 (ANO6)

Alternative Name: ANO6 (ANO6 Products)

Background: ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel,Anoctamin-6 (ANO6, TMEM16F) is a member of the Anoctamin chloride channel family. This family includes 10 proteins: ANO1-ANO10 that are encoded by the conserved TMEM16A-K genes. Anoctamin-6 acts as calcium (Ca2+)-activated chloride (Cl-) channel. It generates an outwardly rectifying Cl- current when activated by a large increase in local intracellular Ca2+ concentrations1. ANO6 structure includes eight transmembrane domains and cytosolic amino- and carboxyl termini. There is a high sequence identity in the putative transmembrane domains and more divergent in other regions2,3.ANO6 has also been shown to operate as a Ca2+-activated phospholipid scramblase resulting in movement of phosphatidylserine from the inner to the outer leaflet of the plasma membrane bilayer. ANO6 is also involved in bone mineralization, cell volume regulation, cell proliferation and apoptosis1,2. ANO6 is expressed in cyst-forming epithelial cells together with ANO1.A mutation in the ANO6 gene is linked to Scott Syndrome, a rare inherited bleeding disorder, characterized by altered Ca2+-dependent platelet signaling with defective phosphatidylserine exposure and microparticle formation. Deletion of ANO6 in mice shows reduced skeleton size, skeletal deformities, and mineralization defects4,5.

Alternative names: Anoctamin-6, ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel

Gene ID: 196527

NCBI Accession: NM_001025356

UniProt: Q4KMQ2

Pathways: SARS-CoV-2 Protein Interactome